Hi,

I'm attempting to stitch a higher-resolution (x60) z-stack of a soma into a lower resolution (x20) stack of the same neuron. A full tiled x60 scan is too large for my hardware to handle, but i need the higher resolution at the soma. I've tried fruitlessly in both FIJI and Vaa3D. Does anyone know a good way of going about this?

P.s. I'm having issues with the newest release of Vaa3D on Sierra 10.12.1. No plugins show up, only the rescan option, which does not work, any ideas?


Thanks, Merlin

You could reconstruct the soma (and nearby processes) from the
60x stack, then separately load the 20x stack and load the
reconstructed soma, then align the soma reconstruction to the 20x
stack and do the rest of your reconstruction from the 20x stack.

What you're trying to do sounds like "multi-scale" image management
(where depending on zoom-level you display/use a higher-resolution
data set, or - if zoomed out - a lower-resolution data set). This is
what e.g. Google Maps does in 2D, with tiles. I don't know whether
Vaa3D can do this, with 3D data.