Personode is a user-friendly, open-source MatLab toolbox that semi-automatically guides through ICA components classification into resting-state networks (RSN), alleviating the selection process, and allows the robust definition of either group- or subject-specific ROIs derived from RSN, which could make network analysis more accurate and individually specific. This tool can produce higher activations than ROIs based on single-studies coordinates or meta-analysis studies for expected regions in a task-related ROI activation analysis and more accurate correlation values (either positive or negative) than clusters belonging to an atlas for expected interactions among networks in a resting state functional connectivity analysis.