Click the “See in 3D” button or follow the main menu “Visualization”, or press “Ctrl-V” (Cmd-V for Mac), or “Shift-V” when you have an image open. Once the 3D viewer is displayed, you can press animation key “Ctrl/Cmd-A” to turn on/off the 3D rotation animation. While you are doing the animation, you can also use volume cut, surface cut, etc.

demo movie on 3D image/surface visualization:

http://penglab.janelia.org/proj/v3d/v3d_...

demo movie on different rendering modes:

http://penglab.janelia.org/proj/v3d/v3d_...