NITRC: Vaa3D and Vaa3D-Neuron: help

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help > Problem with image stack reading by Vaa3D

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Problem with image stack reading by Vaa3D

Dear sir,

I am unable to visualize an image stack properly in Vaa3D. It is unable to register the projections of the neurons through the 3D space. All it sees is the 2D image from the first slice. Is there a possible reason for this problem?

Thanks a lot for your help.

RE: Problem with image stack reading by Vaa3D

In 3D view window, please press Alt + V, and then uncheck "GLSL shader" option.

RE: Problem with image stack reading by Vaa3D

Thanks for your reply. Sorry, I still got a problem. Even after pressing Alt + V, and unchecking "GLSL shader" option, still only the first slice is shown in the 3D viewer. Also, I could not trace the neurons with the App1 because Vaa3D can only see the first slice (which doesn't contain any neurons)...

RE: Problem with image stack reading by Vaa3D

In this way, you may have to use other software(e.g. Fiji) to save the image as v3draw format and the open it in Vaa3D . Or save the image as 2D Image Sequence, and then use Vaa3D to import saved 2D image sequence.

RE: Problem with image stack reading by Vaa3D

Dear Zhi Zhou,

Thanks so much! It worked perfectly.

RE: Problem with image stack reading by Vaa3D

Could you please send your image for us to check? We are very interested in it. Thanks.