help > RE: Strong connections at high thresholds
Apr 29, 2018  02:04 AM | Andrew Zalesky
RE: Strong connections at high thresholds
Hi Katerina,

it is difficult to diagnose this potential problem over a forum. I suggest to undertake some basic sanity checks, such as selecting one of the significant connections and checking whether a significant interaction is still evident when manually computing the ANOVA (i.e. with SPSS or online ANOVA calculator) for the connection.

Or generate plots for the data points corresponding to one of the significant connections and show the 2 groups and 2 points with different colors. Is an interaction visually evident in the plot? If no, then it would seem something has gone wrong.


Originally posted by hex_dex:
Dear Andrew,

You have been very helpful in another topic I had started but I realised that I have wrongly processed my raw data, so I started frpm the beginning. My problem now is the opposite than the last time, I get really strong connections. To be specific, I have 2 groups of interest and 2 time points. I perform a Time x Group analysis using F-test, a=0.001, I test 22 nodes and even at a very high threshold (eg 10) I get really strong connections (eg 18 nodes, 38 edges as in the picture) with p-values equal to zero. I attach a picture of a graph for a specific rhythm tested (a rhythm) and a picture of the fields I fulfill and the results of the p-values. Do you think this is normal?

Additionally when I perform FDR (50000 permutations) I get for the a rhythm results quite similar to that of a threshold=10. Does this mean that everything is ok? I attach the graph for FDR,too.

Last but not least, in order to perform a main effect of time analysis do I need to perform a t-test or am I ok with the f-test?

Thank you,

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hex_dex Apr 28, 2018
hex_dex May 3, 2018
RE: Strong connections at high thresholds
Andrew Zalesky Apr 29, 2018
hex_dex Apr 30, 2018
Andrew Zalesky May 2, 2018
hex_dex Apr 28, 2018
hex_dex Apr 28, 2018