help
help > RE: synapse finding
May 17, 2021 06:05 PM | Ruqiang Liang
RE: synapse finding
HI Yimin,
No, I want to identify and count them from 3D Zstack. Ideally report X, y, Z, and size. Is this possible? I tried with cell_counting plugin from Vaa3D before. Tt was cell_counting -> quickFind -> Exemplar definition and propagation, but not too much success. I guess it depends on the representative of the examples.
Thanks.
Best/ Ruqiang
Originally posted by Yimin Wang:
No, I want to identify and count them from 3D Zstack. Ideally report X, y, Z, and size. Is this possible? I tried with cell_counting plugin from Vaa3D before. Tt was cell_counting -> quickFind -> Exemplar definition and propagation, but not too much success. I guess it depends on the representative of the examples.
Thanks.
Best/ Ruqiang
Originally posted by Yimin Wang:
Hi Ruqiang,
From the snapshot, I assume that the green spots represent synapses and you intend to identify and count hem automatically from a 2D image?
Best,
Yimin
Originally posted by Ruqiang Liang:
From the snapshot, I assume that the green spots represent synapses and you intend to identify and count hem automatically from a 2D image?
Best,
Yimin
Originally posted by Ruqiang Liang:
HI Yimin,
Thank you so much for the swift reply. Here is a snapshot of the result showing in Imaris Viewer. My assistant did this analysis and I just found that it isn't quite accurate. I just want to get the center of each spot labeled by green fluorescence. Any suggestions? Thanks.
Best/ Ruqiang
Thank you so much for the swift reply. Here is a snapshot of the result showing in Imaris Viewer. My assistant did this analysis and I just found that it isn't quite accurate. I just want to get the center of each spot labeled by green fluorescence. Any suggestions? Thanks.
Best/ Ruqiang
Threaded View
| Title | Author | Date |
|---|---|---|
| Ruqiang Liang | May 11, 2021 | |
| Yimin Wang | May 12, 2021 | |
| Ruqiang Liang | May 14, 2021 | |
| Yimin Wang | May 17, 2021 | |
| Ruqiang Liang | May 17, 2021 | |
| Yimin Wang | May 18, 2021 | |