I extracted ROI from wfu_pickatlas toolbox. My data is normalised to EPI template. However, extracted ROI is normalised to some other template.

My question is, how to registered extracted ROI to an EPI template? Is it make sense to normalize ROI two times?

Many thanks in advance,
Very sincerely,
Priya

> dear Priya,
>
> if your data is in a 'normalized EPI template' I think that, in order to
> create the template, you registered all the single-subject data to the MNI
> space. If so, I'm pretty sure that this is the same space in which the ROIs
> of the wfu_pickatlas are provided, therefore, if this is the case, you
> don't need to run any more normalization steps.
>
> If the template is in a space other than the MNI, then you can simply
> register the template to the MNI space, and then use the inverse of the
> transformation matrix to take your ROIs into the template space. I would
> suggest for this last step to use nearest neighbour interpolation (the
> default should be the trilinear).
>
> all the best,
> leonardo

Thank you so much for this clarification.

Although, ROI's dimensions and origin is different from that of my normalized data. Is it ok? As far as i understand, both should be of same dimensions and origin.

Many thanks,
Priya

>


> It looks like you're using SPM, so I'll leave the last word to somebody
> more competent. In general, to perform operations with the ROIs on your
> data (e.g. extract the mean fmri time course in a ROI) both your ROIs and
> data need to have the same dimensions and voxel size.
>

leonardo