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help > RE: BrainAligner
Nov 2, 2017 02:11 AM | Kevin Leo - Harvard Medical School
RE: BrainAligner
Cool! Thank you so much, Yang! This is
very informative. I'll try it by myself first and will let you know
how it goes.
My purpose is to map the expression of several different transcription factors into a single brain. But the problem is that each antibody against different transcription factors has different background. Do you have any suggestions about how to reduce the background?
Another issue of my images is that the signal is getting weak when the location is getting deeper. There is a signal decay along the Z axes. Do you know there are any methods I can use to adjust it?
Many thanks!
My purpose is to map the expression of several different transcription factors into a single brain. But the problem is that each antibody against different transcription factors has different background. Do you have any suggestions about how to reduce the background?
Another issue of my images is that the signal is getting weak when the location is getting deeper. There is a signal decay along the Z axes. Do you know there are any methods I can use to adjust it?
Many thanks!
Threaded View
| Title | Author | Date |
|---|---|---|
| Kevin Leo | Oct 31, 2017 | |
| Kevin Leo | Nov 1, 2017 | |
| Yang Yu | Nov 1, 2017 | |
| Kevin Leo | Nov 2, 2017 | |
| Yang Yu | Nov 2, 2017 | |
| Yang Yu | Oct 31, 2017 | |
| Kevin Leo | Nov 1, 2017 | |