Hi, 

I've been using CMTK to register functional 2-photon calclium imaging stacks of larval zebrafish to a template. While I am in general happy with the registration result, I noticed there are some weird artifcats in the images, where you see a moving stripe or wave of brightness that wasn't there in the original. It's a bit hard to explain so I'll upload a short movie where I go through the stack and put the mouse where this moving stripe appears. I've seen this or similar patterns in three different stacks I've registered and I wanted to find a way to register them without creating this artifact. I tried applying -match-histograms or --histogram-equalization-flt but it didn't change anything. Has this happened to you? Do you have any suggestions on what parameters to tweak to change it?

Thank you, 

Manuel Bettencourt