Gannet is a free, open-source MATLAB-based software toolkit for analyzing edited single-voxel 1H magnetic resonance spectroscopy (MRS) data. Its largely automated functions cover all the essential steps of modern MRS analysis: (1) Loading raw data files; (2) several preprocessing steps; (3) signal modeling; (4) voxel co-registration to and segmentation of structural MR images; and (5) metabolite concentration estimation corrected for tissue composition. Several existing software packages for MRS data analysis require substantial user input or offer a wide selection of processing options. In contrast, the philosophy behind Gannet is to provide users with a complete, automated pipeline that requires minimal user input. Additionally, as open-source software, advanced users can modify the underlying routines for ad hoc purposes.