To visualize / display general surface data (e.g. surfaces, neurons, markers, ...) in V3D is very simple. Just drag and drop the files into the V3D main window or an already opened 3D viewer.

In the former case, a new 3D viewer will be opened for the file.

In the latter case, the file will be opened in the existing 3D viewer and thus you can visualize the newly open data side-by-side with the existing data.

Demo movie: 3D image/surface visualization: http://penglab.janelia.org/proj/v3d/v3d_...