Hi Anita,

Measuring component size with extent or intensity are both valid options and will in most cases yield comparable results. Component size measured using intensity can better capture large effect sizes that are circumscribed to a few connections only. In contrast, component size measured using extent can be more sensitive to spatially diffuse effects. You could try both methods. There is no right or wrong option.

I am not sure if I follow your second question. To visualize the significant network, you will need to provide the MNI coordinates of each node as a text file. Without coordinates, the software does not know where to position the nodes in the brain volume. Of course, you can still run the NBS and generate results without visualizing them, in which case it is not necessary to provide any coordinates. How the ROIs were defined should not affect the running of the NBS. NBS just sees the connectivity matrices for each sample/individual.

Andrew




Originally posted by asinha: