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Jun 1, 2026  03:06 PM | Gabby Guadalupe - Boston Children's Hospital
Error Second Level Analysis

Hello, 


I am trying to calculate resting-state ROI-ROI functional connecvitiy differences between two groups (patients vs. controls), however, I am encountering the following error in Second-Level Analysis:


"Not ready to display second-level Analyses. No conditions found. Please re-run first-level analyses or select different first-level analysis to continue."


In Setup there is only 1 condition (rest), and for 1st-level I select "All conditions" before running.


I reran 1st-level with these parameters and received the same error:



  • Analysis option: ROI-to-ROI anlysis only,

  • correlation bivariate

  • no weighting

  • DMN.MPFC as seed 1, DMN PCC as seed 2

  • no temporal expansion

  • no frequency decomposition


Do I need to change one of the 1st level options or is there another way to complete 2nd level analysis? 


Thank you!  


-Gabby

  Edited (Jun 1, 2026  04:06 PM)
Jun 7, 2026  11:06 AM | Alfonso Nieto-Castanon - Boston University
RE: Error Second Level Analysis

Hi Gabby


Did you get any warning or error message when running those first-level analyses, which may help understand why CONN is not able to proceed to second-level analysis normally? (if in doubt, please send me your conn_*.mat file and I will take a quick look to see if I can find out what may be going on here)


Best


Alfonso


Originally posted by Gabby Guadalupe:



Hello, 


I am trying to calculate resting-state ROI-ROI functional connecvitiy differences between two groups (patients vs. controls), however, I am encountering the following error in Second-Level Analysis:


"Not ready to display second-level Analyses. No conditions found. Please re-run first-level analyses or select different first-level analysis to continue."


In Setup there is only 1 condition (rest), and for 1st-level I select "All conditions" before running.


I reran 1st-level with these parameters and received the same error:



  • Analysis option: ROI-to-ROI anlysis only,

  • correlation bivariate

  • no weighting

  • DMN.MPFC as seed 1, DMN PCC as seed 2

  • no temporal expansion

  • no frequency decomposition


Do I need to change one of the 1st level options or is there another way to complete 2nd level analysis? 


Thank you!  


-Gabby