help > RE: Within-network connectivity after ICA
Jul 22, 2021  12:07 PM | Alfonso Nieto-Castanon - Boston University
RE: Within-network connectivity after ICA
Dear Pravesh,

For within-network connectivity I typically find more convenient to first create meaningful subcomponents of your network and then perform standard ROI-to-ROI analyses on those subcomponents. One simple way to do that is:

step 1) in the Summary tab of the second-level ICA results, select your network(s) of interest, switch the display from "spatial components" to "ICA parcellation" and modify the threshold value there to divide this network into meaningful sub-components, and then select in the ICA tools menu the option "Create ICA parcellation file" to create an ROI file defining those sub-components

step 2) in the Setup.ROIs tab import those new ROI-files as new ROIs for your project

step 3) in the Setup and Denoising tabs click 'Done' and select the 'do not overwrite' option to process only those new ROIs

step 4) in the first-level analysis tab define a new first-level RRC analysis entering only these new ROIs to evaluate the within-network connectivity (e.g bivariate correlations)

Hope this helps
Alfonso

Originally posted by Pravesh Parekh:
Dear Dr. Alfonso/ Conn Experts,

I know that this topic has come up before but perhaps I am missing the relevant posts where the answer is.

I have performed an ICA analysis (20 components) on my sample and have identified a few networks of interest (DMN, FPN, DAN, etc.). Now, I am interested in performing a (voxel-level) within-network analysis for each of the selected network. What would be the best way forward? I see an option of including all the ICA components as masks in the ROI tab (Setup). How should I proceed after that?

P.S: I am using Conn 18b (this is some old analysis; the paper is in revision stage and the reviewer wants us to do some ICA analysis and comment on within-network connectivity of identified components).


Thank you for your time and help

Regards
Pravesh

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TitleAuthorDate
Pravesh Parekh Jul 20, 2021
RE: Within-network connectivity after ICA
Alfonso Nieto-Castanon Jul 22, 2021
Pravesh Parekh Jul 23, 2021