A few things to check

• Make sure that a blank line is not present at end of your design file. This can be interpretted as an additional subject.


• Rather than providing a seperate mat file for each subject, you can concatentate the matricies into a 3D array and provide a single file with that 3D array. It may be that some of the mat files are currently not being read. 


• Ensure that you can run the NBS on the test dataset. If things work on the test dataset, try to decipher what might differ in your dataset. Your design matrix looks fine. I think the issue is a minor formatting bug or file specficiation. 

Best, 

Andrew

Originally posted by Jae Young Park:

Dear NBS forum, 

Hello everyone, I am a student who is currently trying to learn how to use NBS for my research project. I have two groups (22 in total), and I am attempting to perform a two-sample t-test with two covariates to assess group differences in structural connectivity. However, when I run the analysis, NBS returns an error indicating that the connectivity matrix cannot be found or that the connectivity matrices are inconsistent.

I was wondering whether there might be an issue with my connectivity matrix text file, or whether there is another issue causing the error. I have attached my connectivity matrix list (.mat file), design matrix, and contrast file for reference. 

Thank you so much for your time.