Hi Mary,

The mean functional file is typically (when running the default MNI pipeline and using a single T1 file per subject) created during realignment and it represents the mean of all of the functional data (combined across all runs/sessions). This file is saved in the location of the first run functional data just by convention/simplicity, but it nevertheless does reflect the combined data across all runs, not just across the first run.

If, on the other hand, you are using multiple T1 images in order to accommodate possible anatomical changes in the subjects across runs/sessions, then typically the expectation would be to run preprocessing separately for each run/timepoint. If this was your case, then the error that you report would indicate that preprocessing has been run jointly instead of separately across all runs. Since this is a somewhat common point of confusion I am thinking that perhaps I could add something to the preprocessing pipeline that would allow users to easily run preprocessing separately for each session  (perhaps automaticaly switching to this when the "multiple anatomical scans per subject" option is selected?). In any case, let me know your thoughts and I will update this thread when I have these options ready for testing. 

Also, a quick but likely unrelated note: I noticed that one of your files reported in the error message was named auswau101-2_restingstate.nii, the "auswau" prefix often indicates that data already fully preprocessed (e.g. swau101-2_restingstate.nii, with prefix "swau") will have been used as source of yet a new preprocessing pipeline (hence the new "au" prefixes added). Please double-check and remember to reset your functional and structural tabs to their original states (raw/original data, probably the files "101-2_restingstate.nii" in the example above) before re-running preprocessing. 

Best

Alfonso

Originally posted by Mary Newsome:

Hi Alfonso and everyone,

We have a Pre-Post design where each subject has 2 sessions. I'm wondering why there is a mean file created for the first session, but not for the second.

During Preprocessing for the second session, I'm getting error messages:  "Mean functional file not found..." and

"/raw_101-2/Resting_State_Analysis_Directory/auswau101-2_restingstate.nii ; run either realignment or ART first, or select the option "first functional
volume as reference" -#coregtomean field- if you prefer to use the first functional volume instead of the mean functional volume as reference)"

Thanks for any help!

Best,

Mary