open-discussion > Need help for fcon 1000 preprocessing
Aug 16, 2011  11:08 AM | makiko yamada
Need help for fcon 1000 preprocessing
Hello,

I am using fcon_1000 for rsfMRI processing for the first time.
My environment is Mac OSX10.7.

I ran the batch_process.sh script, but it ended with errors as below. I cannot figure out how to solve these errors.
Could you please help me out?

Makiko


[macpro101208:~/fcon_1000/scripts] yamadamakiko% ./batch_process.sh
/Users/yamadamakiko/fcon_1000/EXP02 + /Users/yamadamakiko/fcon_1000/EXP02/EXP02_subjects.txt + 4 + 203 + 200 + 2
preprocessing sub001
--------------------------------------
!!!! PREPROCESSING ANATOMICAL SCAN!!!!
--------------------------------------
deobliquing sub001 anatomical
++ 3drefit: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
++ Authored by: RW Cox
++ Processing AFNI dataset mprage.nii.gz
 + loading and re-writing entire dataset mprage.nii.gz
++ 3drefit processed 1 datasets
Reorienting sub001 anatomical
skull stripping sub001 anatomical
dyld: Library not loaded: /sw/lib/libglib-1.2.0.dylib
  Referenced from: /Users/yamadamakiko/abin/3dSkullStrip
  Reason: image not found
/Users/yamadamakiko/fcon_1000/scripts/1_anatpreproc.sh: line 45: 52304 Trace/BPT trap: 5       3dSkullStrip -input ${anat}_RPI.nii.gz -o_ply ${anat}_surf.nii.gz
++ 3dcalc: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
++ Authored by: A cast of thousands
** FATAL ERROR: can't open dataset mprage_surf.nii.gz
** Program compile date = Aug 12 2011
---------------------------------------
!!!! PREPROCESSING FUNCTIONAL SCAN !!!!
---------------------------------------
Dropping first TRs
++ 3dcalc: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
++ Authored by: A cast of thousands
++ Output dataset ./rest_dr.nii.gz
Deobliquing sub001
++ 3drefit: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
++ Authored by: RW Cox
++ Processing AFNI dataset rest_dr.nii.gz
 + loading and re-writing entire dataset rest_dr.nii.gz
++ 3drefit processed 1 datasets
Reorienting sub001
Motion correcting sub001
++ 3dTstat: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
++ Authored by: KR Hammett & RW Cox
++ Output dataset ./rest_ro_mean.nii.gz
++ 3dvolreg: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
++ Authored by: RW Cox
++ Coarse del was 10, replaced with 3
++ Max displacement in automask = 1.11 (mm) at sub-brick 0
Skull stripping sub001
++ 3dAutomask: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
++ Authored by: Emperor Zhark
++ Loading dataset rest_mc.nii.gz
++ Forming automask
 + Fixed clip level = 1825.005005
 + Used gradual clip level = 1599.834961 .. 2094.415283
 + Number voxels above clip level = 25734
 + Clustering voxels ...
 + Largest cluster has 25586 voxels
 + Clustering voxels ...
 + Largest cluster has 24864 voxels
 + Filled   187 voxels in small holes; now have 25051 voxels
 + Clustering voxels ...
 + Largest cluster has 25051 voxels
 + Clustering non-brain voxels ...
 + Clustering voxels ...
 + Largest cluster has 118309 voxels
 + Mask now has 25051 voxels
++ Dilating automask
 + Clustering voxels ...
 + Largest cluster has 113653 voxels
++ 29707 voxels in the mask [out of 143360: 20.72%]
++ first  14 x-planes are zero [from R]
++ last   13 x-planes are zero [from L]
++ first   6 y-planes are zero [from P]
++ last    8 y-planes are zero [from A]
++ first   0 z-planes are zero [from I]
++ last    2 z-planes are zero [from S]
++ Output dataset ./rest_mask.nii.gz
++ CPU time = 0.800000 sec
++ 3dcalc: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
++ Authored by: A cast of thousands
++ Output dataset ./rest_ss.nii.gz
Getting example_func for registration for sub001
++ 3dcalc: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
++ Authored by: A cast of thousands
++ Output dataset ./example_func.nii.gz
Smoothing sub001
/Users/yamadamakiko/fcon_1000/scripts/2_funcpreproc.sh: line 75: fslmaths: command not found
Grand-mean scaling sub001
/Users/yamadamakiko/fcon_1000/scripts/2_funcpreproc.sh: line 79: fslmaths: command not found
Band-pass filtering sub001
++ 3dFourier: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
** FATAL ERROR: Can't open dataset 'rest_gms.nii.gz'
** Program compile date = Aug 12 2011
Removing linear and quadratic trends for sub001
++ 3dTstat: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
++ Authored by: KR Hammett & RW Cox
** FATAL ERROR: Can't open dataset rest_filt.nii.gz
** Program compile date = Aug 12 2011
++ 3dDetrend: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
** FATAL ERROR: Can't open dataset 'rest_filt.nii.gz'
** Program compile date = Aug 12 2011
++ 3dcalc: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
++ Authored by: A cast of thousands
** FATAL ERROR: can't open dataset rest_filt_mean.nii.gz
** Program compile date = Aug 12 2011
Generating mask of preprocessed data for sub001
/Users/yamadamakiko/fcon_1000/scripts/2_funcpreproc.sh: line 93: fslmaths: command not found
------------------------------
!!!! RUNNING REGISTRATION !!!!
------------------------------
cp: /Users/yamadamakiko/fcon_1000/EXP02/sub001/anat/mprage_brain.nii.gz: No such file or directory
/Users/yamadamakiko/fcon_1000/scripts/3_registration.sh: line 57: flirt: command not found
/Users/yamadamakiko/fcon_1000/scripts/3_registration.sh: line 59: convert_xfm: command not found
/Users/yamadamakiko/fcon_1000/scripts/3_registration.sh: line 63: flirt: command not found
/Users/yamadamakiko/fcon_1000/scripts/3_registration.sh: line 65: convert_xfm: command not found
/Users/yamadamakiko/fcon_1000/scripts/3_registration.sh: line 69: convert_xfm: command not found
/Users/yamadamakiko/fcon_1000/scripts/3_registration.sh: line 71: flirt: command not found
/Users/yamadamakiko/fcon_1000/scripts/3_registration.sh: line 73: convert_xfm: command not found
/Users/yamadamakiko/fcon_1000/scripts/tissuepriors/3mm/
------------------------------
!!!! RUNNING SEGMENTATION !!!!
------------------------------
Segmenting brain for sub001
/Users/yamadamakiko/fcon_1000/scripts/4_segment.sh: line 55: fast: command not found
Creating global mask
++ 3dcopy: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
** failed to open input '/Users/yamadamakiko/fcon_1000/EXP02/sub001/func/rest_pp_mask.nii.gz' on non-AFNI write
Try including the view of the input dataset /Users/yamadamakiko/fcon_1000/EXP02/sub001/func/rest_pp_mask.nii.gz
or use its fullname. That might take care of the error.
Registering sub001 csf to native (functional) space
/Users/yamadamakiko/fcon_1000/scripts/4_segment.sh: line 64: flirt: command not found
Smoothing sub001 csf
/Users/yamadamakiko/fcon_1000/scripts/4_segment.sh: line 67: fslmaths: command not found
Registering sub001 csf to standard space
/Users/yamadamakiko/fcon_1000/scripts/4_segment.sh: line 70: flirt: command not found
Finding overlap between sub001 csf and prior
/Users/yamadamakiko/fcon_1000/scripts/4_segment.sh: line 73: fslmaths: command not found
Registering sub001 csf back to native space
/Users/yamadamakiko/fcon_1000/scripts/4_segment.sh: line 76: flirt: command not found
Threshold and binarize sub001 csf probability map
/Users/yamadamakiko/fcon_1000/scripts/4_segment.sh: line 79: fslmaths: command not found
Mask csf image by sub001 functional
/Users/yamadamakiko/fcon_1000/scripts/4_segment.sh: line 82: fslmaths: command not found
Registering sub001 wm to native (functional) space
/Users/yamadamakiko/fcon_1000/scripts/4_segment.sh: line 87: flirt: command not found
Smoothing sub001 wm
/Users/yamadamakiko/fcon_1000/scripts/4_segment.sh: line 90: fslmaths: command not found
Registering sub001 wm to standard space
/Users/yamadamakiko/fcon_1000/scripts/4_segment.sh: line 93: flirt: command not found
Finding overlap between sub001 wm and prior
/Users/yamadamakiko/fcon_1000/scripts/4_segment.sh: line 96: fslmaths: command not found
Registering sub001 wm back to native space
/Users/yamadamakiko/fcon_1000/scripts/4_segment.sh: line 99: flirt: command not found
Threshold and binarize sub001 wm probability map
/Users/yamadamakiko/fcon_1000/scripts/4_segment.sh: line 102: fslmaths: command not found
Mask wm image by sub001 functional
/Users/yamadamakiko/fcon_1000/scripts/4_segment.sh: line 105: fslmaths: command not found
--------------------------------------------
!!!! RUNNING NUISANCE SIGNAL REGRESSION !!!!
--------------------------------------------
Splitting up sub001 motion parameters
Extracting global signal for sub001
++ 3dmaskave: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
*** Cannot open mask dataset!
Extracting signal from csf for sub001
++ 3dmaskave: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
*** Cannot open mask dataset!
Extracting signal from white matter for sub001
++ 3dmaskave: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
*** Cannot open mask dataset!
Modifying model file
Running feat model
/Users/yamadamakiko/fcon_1000/scripts/5_nuisance.sh: line 82: feat_model: command not found
** FATAL ERROR: Can't open dataset '/Users/yamadamakiko/fcon_1000/EXP02/sub001/func/rest_pp_mask.nii.gz'
** Program compile date = Aug 12 2011
Running film to get residuals
/Users/yamadamakiko/fcon_1000/scripts/5_nuisance.sh: line 88: film_gls: command not found
++ 3dTstat: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
++ Authored by: KR Hammett & RW Cox
** FATAL ERROR: Can't open dataset /Users/yamadamakiko/fcon_1000/EXP02/sub001/func/nuisance/stats/res4d.nii.gz
** Program compile date = Aug 12 2011
++ 3dcalc: AFNI version=AFNI_2011_05_26_1457 (Aug 12 2011) [64-bit]
++ Authored by: A cast of thousands
** FATAL ERROR: can't open dataset /Users/yamadamakiko/fcon_1000/EXP02/sub001/func/nuisance/stats/res4d.nii.gz
** Program compile date = Aug 12 2011
/Users/yamadamakiko/fcon_1000/scripts/5_nuisance.sh: line 95: flirt: command not found

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TitleAuthorDate
Need help for fcon 1000 preprocessing
makiko yamada Aug 16, 2011
John Magnotti Aug 16, 2011
Maarten Mennes Aug 16, 2011
makiko yamada Aug 17, 2011
John Magnotti Aug 17, 2011
makiko yamada Aug 18, 2011
John Magnotti Aug 18, 2011
makiko yamada Aug 19, 2011
John Magnotti Aug 19, 2011