First, it is unclear what you meant by "4D". There are several cases.

(1) If the 4D means X,Y,Z,C where C is color channel, then you can simply visualize in a 3D view window. However, when C is greater than 3, you will need a blending function. In Vaa3D theer are several ways to do that, e.g. the atlas manager.

(2) If 4D means X,Y,Z, and T, where T is time, then you can use the 3D viewer to see it, the time scrollbar will appear at the bottom of a 3D viewer.

(3) If 4D means X,Y, C and T, then similar to (2), you can use the time scroll bar to see the data.

Second, if your files have the sequence like xxx_01.tif, xxx_02.tif, ... instead of using the 5D stack converter, you can import the sequence using Main Menu -> File -> Import -> Impotrnt general Image Series (the first menu item i=there). Then if your data is temporal data , then select the bottom option in the pop-up dialog to be "Pack in Channel". Then when you produce the 3D viewer, you can see the temporal scroll bar, while you are visualizing data in 3D.

-Hanchuan



Originally posted by Crystal Davey: