Ok, normally we store a 3D image stack, which includes X,Y,Z, into one SINGLE file. This can be stored as 3D TIFF format, or as a Vaa3D's Raw file. Each file can also has a color channel (which will make it actually a 4D image stack).


Then, each time point corresponds to a single image stack file. Each image stack has the naming convention xxx_001.tif, xxx_002.tif, ...

Then use the import function in my last msg to load the data into "Color Channel". Suppose you have 100 time points, each is a 4D image stack of the size 512 x 300 x 400 x 2 (in the order of X, Y, Z, and C). Then after importing, in the tri-view main window you will see the loaded data has 2*100 = 200 color channels. Ignore that, as the software actually knows the loaded data has 100 time points! Then when you open the 3D viewer window, you can see the 3D rendered data has 100 time points and 2 color channels, and 400 z-slices!

-Hanchuan


Originally posted by Crystal Davey: