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users > No reformatted files produced from Fiji/CMTK registration
Jun 28, 2019 03:06 PM | Zane Aldworth
No reformatted files produced from Fiji/CMTK registration
Hi all,
I recently tried to use the CMTK gui from FIJI in order to register some brains. While the process seemed to work well and generated a "Registration" directory (with affine and warp subdirectories), I did not see a directory for the reformatted images, nor indeed any reformatted images in the other directories. I did find the "registration" output file, which I gather I could use to transform the original images if I wanted to install command line CMTK, but that seems to be a bit against the spirit the spirit of using a gui.
I did check (and uncheck and re-check) the box for reformat in the gui, and I performed the registration with affine & warp as well as affine only, with all output results being similar- registration folder produced, but no reformatted image.
Should I expect reformatted images as an output of FIJI/CMTK?
Also, assuming I can get the reformatted images, I have a couple of further options from command line CMTK that I would like to implement in the gui, but it seems that the syntax may need to be a bit different:
I'm registering labeled files instead of actual brains, so I need to add the "--class-ref label" and "--class-flt label" tags- is that the proper syntax? And would assume it goes to the "further registration params" field?
Similarly, I believe I read that the default similarity measure is nmi. Is that the case, and if not can I just add "--nmi"?
What is the default interpolation method, and how could I change it to partial volume (assuming "--partial-volume" doesn't work)?
For the affine registration I see 9 degrees of freedom as output, but haven't seen a 12 degree of freedom output (i.e. no sheer elements in the "registration" output file). Is there a way to do sheering or to specify the dof in the registration?
Finally, once the labelled files have been registered, I would like to apply the transformation matrices to both the original image stacks as well as to a traced neuron file. Are there commands equivalent dof2mat and reformatx from the command line?
Thanks for any help!
I recently tried to use the CMTK gui from FIJI in order to register some brains. While the process seemed to work well and generated a "Registration" directory (with affine and warp subdirectories), I did not see a directory for the reformatted images, nor indeed any reformatted images in the other directories. I did find the "registration" output file, which I gather I could use to transform the original images if I wanted to install command line CMTK, but that seems to be a bit against the spirit the spirit of using a gui.
I did check (and uncheck and re-check) the box for reformat in the gui, and I performed the registration with affine & warp as well as affine only, with all output results being similar- registration folder produced, but no reformatted image.
Should I expect reformatted images as an output of FIJI/CMTK?
Also, assuming I can get the reformatted images, I have a couple of further options from command line CMTK that I would like to implement in the gui, but it seems that the syntax may need to be a bit different:
I'm registering labeled files instead of actual brains, so I need to add the "--class-ref label" and "--class-flt label" tags- is that the proper syntax? And would assume it goes to the "further registration params" field?
Similarly, I believe I read that the default similarity measure is nmi. Is that the case, and if not can I just add "--nmi"?
What is the default interpolation method, and how could I change it to partial volume (assuming "--partial-volume" doesn't work)?
For the affine registration I see 9 degrees of freedom as output, but haven't seen a 12 degree of freedom output (i.e. no sheer elements in the "registration" output file). Is there a way to do sheering or to specify the dof in the registration?
Finally, once the labelled files have been registered, I would like to apply the transformation matrices to both the original image stacks as well as to a traced neuron file. Are there commands equivalent dof2mat and reformatx from the command line?
Thanks for any help!
Threaded View
Title | Author | Date |
---|---|---|
Zane Aldworth | Jun 28, 2019 | |
Greg Jefferis | Jun 28, 2019 | |
Zane Aldworth | Jun 28, 2019 | |
Greg Jefferis | Jun 29, 2019 | |
Zane Aldworth | Jun 30, 2019 | |
Greg Jefferis | Jun 30, 2019 | |
Jonathan Perdomo | Dec 17, 2020 | |
Greg Jefferis | Dec 17, 2020 | |
Jonathan Perdomo | Dec 18, 2020 | |