NeuroDOT, a MATLAB- and Python- based self-contained toolbox, addresses common challenges in processing of functional near infrared spectroscopy (fNIRS) and diffuse optical tomography (DOT) data. NeuroDOT supports multiple common pre-processing and analytical pipelines for simulation, data-anatomy alignment and modeling, pre-processing, data quality control, reconstruction, spectroscopy, post-processing, and extensive data visualizations at all stages of processing. We also provide pre-assembled, anonymized, and published data samples from our lab to reflect common experimental paradigms in neuroimaging including visual, language, and resting tasks for human brain mapping. Visualization and analysis tools provide powerful and intuitive explorations of data and data quality. Register to receive development updates, news about workshops, and individual help from the NeuroDOT team: https://tinyurl.com/NeuroDOT . The latest release version of NeuroDOT is v1.5 (May 22 2025).