Hi Alfonso,

Thank you for the very helpful response! Is it also possible to set up the second-level analysis with three "groups": the "pre" time point, "post" time point, and time points from healthy controls (while modeling the control group as zero)? With this setup, I believe the necessary variance would be obtained in order to perform the connectivity analysis with only one subject - is that correct? If so, how would I set this up?

Thanks!
Kaitlin